Work Package 3 will accelerate the discovery and deployment of genes and alleles of high value for breeding, thus maximising impact from discoveries within Work Package 1 and 2, 20:20 Wheat and John Innes Centre’s 2012-17 Wheat Institute Strategic Programme.
Tangible Work Package 3 outputs for this purpose are referred to as ‘toolkits’, one composed of pre-breeding germplasm and the other the molecular markers needed to exploit that germplasm. Work Package 3 addresses two topics. The first advances the genetic and genomic characterisation of foundation germplasm. This germplasm, together with the derived segregating populations and associated methodologies, will supply new toolkit genes, alleles, and markers.
In the second the new genes, alleles and markers are validated and tested for their value in breeding. This gene discovery funnel produces packages of traits with molecular markers assembled into the two toolkits. Work Package 3 channels new genes and alleles based on the discoveries of DFW and previous wheat programme outputs, into commercial wheat breeding.
Accessions of the AE Watkins landrace collection were collected from markets from 32 countries across the world. From the over 800 lines of this collection a core set of 119 accessions has been selected, which captures the majority of the genetic diversity (Wingen et al, TAG, 2014).
Information on accession names, country of origin, growth habit and ancestral group can be downloaded here:
The bread wheat landrace NAM (nested association mapping) panel was developed as a set of bi-parental segregating F4 single-seed populations from crosses of spring wheat accession ‘Paragon’ with diverse wheat germplasm, particularly from the AE Watkins core set. Population development was conducted within the WGIN and the WISP project. As of January 2016 there were 127 Watkins populations and many Gediflux populations under development.
Populations were genotyped, mostly employing KASPar SNP markers, and genetic maps were developed (Wingen et al. Genetics 2017). In total, 90 bi-parental population maps are currently available:
- KASP genetic maps of 87 populations from the Bread Wheat Landrace NAM Panel
- Axiom/iSelect genetic maps of 3 populations from the Bread Wheat Landrace NAM Panel
Near Isogenic Lines (NILs), developed from ParagonXWatkins populations in Paragon background, were genotyped with the 35k bread wheat axiom chip and the markers arranged along the whole genome assembly. The graphical genotypes can be inspected on the following webpages:
Phenotypic data from JIC Church Farm field trials is available as spread sheets for different resources:
A changing set of NAM populations is grown every year in field trials at at JIC:
- 2011 JIC trait data from 7 of the landrace NAM populations
- 2012 JIC trait data from 2 of the landrace NAM populations
- 2013 JIC trait data from 6 of the landrace NAM populations
- 2014 JIC trait data from 26 of the landrace NAM populations
- 2015 JIC trait data from 17 of the landrace NAM populations
- 2016 JIC trait data from 6 of the landrace NAM populations
- 2017 JIC trait data from 9 of the landrace NAM populations
This resource has a dedicated page here
The non-restrictive Material Transfer Agreement needed to use our resources is available here: MTA template