Designing Future Wheat Resources
Breeders Diversity Toolkit
- The ‘Recommended List’ of DFW pre breeding lines.
- Near Isogenic Lines under constant selection.
- Best available molecular markers for target QTL.
- The product delivered by DFW to commercial wheat breeding.
- Led to the formation of a new post- the Derived Germplasm Specialist at the GRU
The aim of Designing Future Wheat (DFW) Programme is to deliver new useful genetic variation and knowledge to breeding. Pre-breeding germplasm is developed and handled in a collaboration between DFW WP3 and the Germplasm Resource Unit (GRU). The new pre-breeding germplasm will include near isogenic lines (NILs) from wheat diversity sources. NILs or equivalent material are assessed for the trait of interest within DFW.
Particularly promising germplasm will be multiplied and trialed in multi-site trials at commercial breeders and scientific institutions as an annually changing Breeders’ Diversity Toolkit (BTK) series. The DFW BTK series carries on from the WISP Breeders’ Diversity Toolkit (see below). It is one major result of DFW, taking output from WP1 and WP2 forward to pre-breeding.
DFW Breeders’ Diversity Toolkit Selection Committee
Decisions on QTL or genomic regions and germplasm are made by the Breeders Toolkit Annual Selection Committee Meeting. It decides on the content of the DFW NIL back-crossing pipeline (NIL sets, putative candidates for a BTK) and on the final selection of the annual BTK lines.
The following representatives sit on the committee:
QTL or genomic regions and germplasm can be nominated by any researcher within DFW to the chair of the committee by submitting a completed form on germplasm, trait of interest, markers etc. using the following form: BTK germplasm nomination form. The committee meets every year in February to decide on the larger back-crossing pipeline and in September to decide on the next BTK.
The BTK will be tested in multi-site trials at different breeding companies and institutions.
Summary of all accessions grown in BTK trials from 2017-2022
Download a spreadsheet with the accession names from all Breeders’ Toolkit trials up to 2022:
DFW Breeders’ Toolkit (BTK) 2021-2022
The accessions in the September 2021 selection are: a) introgressions of Synthetic Wheat into Robigus (WS); b) BC3 Near Isogenic Lines from mutant wheat into Paragon (WM); c) introgressions of Ae. Mutica (wild wheat relative) into Paragon (WR); e) Near Isogenic Lines from landrace cultivars in Paragon (WL). Not all lines are grown at all seven Breeders’ sites. Accessions and controls were planted in mostly three replicates in yield trials.
Type (Wheat) |
Accession Name |
Description |
Recipent Cultivar |
BTK Code |
Breed. sites |
WS | SHW095-SEL 0454 | donor NIAB SHW095 | Robigus | WBTK0049 | 7 |
WS | SHW035-SEL 0302 | donor NIAB SHW035 | Robigus | WBTK0050 | 7 |
WS | SHW089-SEL 0385 | donor NIAB SHW089 | Robigus | WBTK0051 | 7 |
WS | SHW027-SEL 0279 | donor NIAB SHW027 | Robigus | WBTK0052 | 7 |
WS | SHW080-SEL 0435 | donor NIAB SHW080 | Robigus | WBTK0053 | 7 |
WS | SHW082-SEL 0338 | donor NIAB SHW082 | Robigus | WBTK0054 | 7 |
WM | GPC-B1_WT GW2-ABD_WT | WT (BC3) | Paragon | WBTK0055 | 4 |
WM | gpc-B1_Mut GW2-ABD_WT | GPC-B1 funct | Paragon | WBTK0056 | 7 |
WM | GPC-B1_WT gw2-ABD_Mut | gw2-triple + GPC-B1 non-funct | Paragon | WBTK0057 | 4 |
WM | gpc-B1_Mut gw2-ABD_Mut | gw2-triple + GPC-B1 funct | Paragon | WBTK0058 | 7 |
WR | NU DHF1-006 T.ae. x Ae. mutica | BC DH | Paragon | WBTK0059 | 7 |
WR | NU DHF1-011 T.ae. x Ae. mutica | BC DH | Paragon | WBTK0060 | 4 |
WR | NU DHF1-010 T.ae. x Ae. mutica | BC DH | Paragon | WBTK0061 | 4 |
WR | NU DHF1-008 T.ae. x Ae. mutica | BC DH | Paragon | WBTK0062 | 4 |
WR | NU DHF1-013 T.ae. x Ae. mutica | BC DH | Paragon | WBTK0063 | 4 |
WL | ParW292-54-8-2-39-Q5B-PH-W | Wat292 QTL 5B-PH | Paragon | WBTK0064 | 4 |
WL | ParW292-54-8-2-43-Q5B-PH-P | Wat292 QTL 5B-PH | Paragon | WBTK0065 | 4 |
WL | ParW475-31-4-6-3-Q5B-GW-W | Wat475 QTL 5B-GW | Paragon | WBTK0066 | 4 |
WL | ParW475-31-4-6-15-Q5B-GW-P | Wat475 QTL 5B-GW | Paragon | WBTK0067 | 4 |
WL | ParW387-15-13-2-6-Q4A-GW-W | Wat387 QTL 4A-GW | Paragon | WBTK0068 | 4 |
WL | ParW387-15-13-2-16-Q4A-GW-P | Wat387 QTL 4A-GW | Paragon | WBTK0069 | 4 |
WL | ParW496-41-12-4-14-Q2D-PH-W | Wat496 QTL 2D-PH | Paragon | WBTK0070 | 4 |
WL | ParW496-41-12-4-7-Q2D-PH-P | Wat496 QTL 2D-PH | Paragon | WBTK0071 | 4 |
WL | ParW141-4-5-1-15-Q6B-GY-W | Wat141 QTL 6B-GY | Paragon | WBTK0072 | 4 |
WL | ParW141-4-5-1-2-Q6B-GY-P | Wat141 QTL 6B-GY | Paragon | WBTK0073 | 4 |
WL | ParW199-6-9-6-21-Q6B-NDVI-W | Wat199 QTL 6B-NDVI | Paragon | WBTK0074 | 4 |
WL | ParW199-6-9-6-38-Q6B-NDVI-P | Wat199 QTL 6B-NDVI | Paragon | WBTK0075 | 4 |
WL | ParW032-78-7-14-10-Q4D-GY-W | Wat032 QTL 4D-GY | Paragon | WBTK0076 | 4 |
WL | ParW032-78-7-14-11-Q4D-GY-P | Wat032 QTL 4D-GY | Paragon | WBTK0077 | 4 |
WL | ParW468-34-12-7-19-Q6A-HI-W | Wat468 QTL 6A-HI | Paragon | WBTK0078 | 1 |
WL | ParW468-34-12-7-5-Q6A-HI-P | Wat468 QTL 6A-HI | Paragon | WBTK0079 | 1 |
WL | ParW199-22-5-4-10-Q2B-HI-W | Wat199 QTL 2B-HI | Paragon | WBTK0080 | 1 |
WL | ParW199-22-5-4-15-Q2B-HI-P | Wat199 QTL 2B-HI | Paragon | WBTK0081 | 1 |
WL | ParW034-13-2-7-17-Q2A-BM-W | Wat034 QTL 2A-BM | Paragon | WBTK0082 | 1 |
WL | ParW034-13-2-7-22-Q2A-BM-P | Wat034 QTL 2A-BM | Paragon | WBTK0083 | 1 |
WL | ParW398-88-13-5-9-Q6B-Hd-W | Wat398 QTL 6B-Hd | Paragon | WBTK0084 | 1 |
WL | ParW398-88-13-5-15-Q6B-Hd-P | Wat398 QTL 6B-Hd | Paragon | WBTK0085 | 1 |
WL | ParW209-42-4-2-16-Q6A-BM-W | Wat209 QTL 6A-BM | Paragon | WBTK0086 | 1 |
WL | ParW209-42-4-2-13-Q6A-BM-P | Wat209 QTL 6A-BM | Paragon | WBTK0087 | 1 |
WL | ParW292-91-1-3-18-Q3D-BM-W | Wat292 QTL 3D-BM | Paragon | WBTK0088 | 1 |
WL | ParW292-91-1-3-11-Q3D-BM-P | Wat292 QTL 3D-BM | Paragon | WBTK0089 | 1 |
WL | ParW246-83-4-5-7-Q1B-NDVI-W | Wat246 QTL 1B-NDVI | Paragon | WBTK0090 | 1 |
WL | ParW246-83-4-5-15-Q1B-NDVI-P | Wat246 QTL 1B-NDVI | Paragon | WBTK0091 | 1 |
WL | ParW199-59-6-8-12-Q7A-BM-W | Wat199 QTL 7A-BM | Paragon | WBTK0092 | 1 |
WL | ParW199-59-6-8-10-Q7A-BM-P | Wat199 QTL 7A-BM | Paragon | WBTK0093 | 1 |
Control | Paragon | Spring Control | WBTK-PAR1 | 7 | |
Control | Robigus | Winter Control | WBTK-ROB1 | 7 | |
Control | Santiago | Winter Control | WBTK-SAN1 | 7 |
DFW Breeders’ Toolkit (BTK) 2020-2021
Like last season, the accessions in the September 2020 selection: are a) introgressions of Durum, Emmer or Synthetic Wheat into Robigus (WS); b) one introgressions of Ae. Mutica (wild wheat relative) into Paragon (WR); c) Near Isogenic Lines from landrace cultivars in Paragon (WL); or e) Near Isogenic Lines from mutant wheat into Paragon (WM). Accessions and controls were planted in 3 replicates in yield trials.
Type (Wheat) |
Accession Name |
Description |
Recipent Cultivar |
GRU Code |
WS |
SHW-075Bc1 F6_SEL165 |
SHW-075Bc1 F6_SEL165 |
Robigus |
WBTK0041 |
WS |
SHW-008Bc1 F7_SEL63 |
SHW-008Bc1 F7_SEL63 |
Robigus |
WBTK0040 |
WS |
SHW-008Bc1 F7_SEL57 |
SHW-008Bc1 F7_SEL57 |
Robigus |
WBTK0039 |
WS |
SHW-008Bc1 F7_SEL50B |
SHW-008Bc1 F7_SEL50B |
Robigus |
WBTK0038 |
WS |
DFW SEL 0011B |
DFW SEL 0011B |
Robigus |
WBTK0036 |
WS |
DFW SEL 0015C |
DFW SEL 0015C |
Robigus |
WBTK0036 |
WR |
DHF1-196 T. aestivum x Ae. Mutica |
Ae. mutica DH BC |
Paragon |
WBTK0046 |
WL |
PW292-93-7-13-Q1B-MATU-W |
Wat 1190292 QTL 1B-MAT |
Paragon |
WBTK0045 |
WL |
PW292-93-7-16-Q1B-MATU-P |
Wat 1190292 QTL 1B-MAT |
Paragon |
WBTK0044 |
WL |
PW352-26-2-19-Q5D-MATU-P |
Wat 1190352 QTL 5D-MAT |
Paragon |
WBTK0042 |
WL |
PW352-26-2-2-Q5D-MATU-W |
Wat 1190352 QTL 5D-MAT |
Paragon |
WBTK0043 |
WM |
Paragon (BC4) (gw2-ABD_Mut) |
TaGW2 triple knock-out |
Paragon |
WBTK0047 |
WM |
Paragon (BC4) (GW2_ABD_WT) |
TaGW2 wild-type |
Paragon |
WBTK0048 |
Control |
Paragon |
Spring Control |
W10074 |
|
Control |
Santiago |
Winter Control, Yield Standard |
W10338 |
|
Control |
Robigus |
Winter Control |
W9999 |
BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU – BTK collection), Par=Paragon wild type, Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic.
DFW Breeders’ Toolkit (BTK) 2019-2020
The accessions in the September 2019 selections are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus; or introgressions of wild wheat relatives into Paragon; or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls were planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2019-20:
Type (Wheat) |
Accession Name |
Description of Introgression |
Recipient Cultivar |
GRU code |
WL | PW141-21-3-4-Q2A-GFP-W | Wat 1190141 QTL 2A-GFP | Paragon | WBTK0020 |
WL | PW292-25-6-3-Q2A-MATU-W | Wat 1190292 QTL 2A-MATU | Paragon | WBTK0021 |
WL | PW352-26-5-19-Q2A-DTMA-W | Wat 1190352 QTL 2A-DTMA | Paragon | WBTK0022 |
WL | PW468-84-4-2-Q5A-NDRE-W | Wat 1190468 QTL 5A-NDRE | Paragon | WBTK0023 |
WS | SHW008 SYN Sel 51 | NIAB SHW-008 BC1F6 | Robigus | WBTK0024 |
WS | SHW008 SYN Sel 61 | NIAB SHW-008 BC1F6 | Robigus | WBTK0025 |
WS | SHW090 SYN Sel 70 | NIAB SHW-090 BC1F6 | Robigus | WBTK0026 |
WR | D029 DHF1-29 | Ae. mutica DH BC | Paragon | WBTK0027 |
WR | D329 DHF1-329 | Ae. mutica DH BC | Paragon | WBTK0028 |
WM | gw2-A / Par (+/-) | gw2-A | Paragon | WBTK0029 |
WM | Par / POL (-/+) | POL | Paragon | WBTK0030 |
WM | gw2-A / POL (+/+) | gw2-A and POL | Paragon | WBTK0031 |
Control | Paragon Parent | Spring Control | W10074 | |
Control | Robigus Parent | Winter Control | W9999 | |
Control | Santiago Yield Standard | Winter Control | W10338 |
Abbreviations:
BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU – BTK collection), gw2-A= grain weigth 2-A allele, Par=Paragon wild type, POL=T. polonicum P1 allele (grain length), Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic .
DFW Breeders’ Toolkit 2018-2019
The accessions in the current selection (September 2018) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2018 (GRU codes in brackets):
Accession | Description | GRU code |
---|---|---|
NIAB-TK-1746 | Karim Durum Wheat introgression into Paragon | WS0087 |
NIAB-TK-1549: | Synthetic Hexaploid Wheat-18 introgression into Robigus | WS0063 |
NIAB-TK-1604: | Tios Emmer Landrace introgression into Robigus | WS0076 |
PW34.12 Q1A-HT | landrace allele from Watkins 1190034 | WL0105 |
PW34.12 Q1A-HT | comparative Paragon allele | WL0104 |
PW468.84 Q5A GRYLD | landrace allele from Watkins 1190468 | WL0292 |
PW468.84 Q5A GRYLD | comparative Paragon allele | WL0291 |
Paragon, | parental control | |
Robigus, | parental control | |
Santiago, | industry standard |
DFW Breeders’ Diversity Toolkit 2017
The genotypes in the current selection (September 2017) are Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).
- Information and statistics on the result of DFW BTK 2017 will be published here soon after harvest 2018.
- Information and statistics on the result of WISP BTK 2016 and the new varieties in the DFW 2017 Toolkit: DFW_WP3_Breeders_Toolkit_2017.pdf
To obtain seed contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at simon.orford@jic.ac.uk
Normally, 500g of each line requested will be dispatched upon receipt of signed MTA. In the event of seed stock exhaustion, seed will be provided after regeneration at GRU.
Axiom Genotypes of NILs
Many of the NILs, candidates for the BTK, have been genotyped using the 35k bread wheat genotyping array. Genotypes can be inspected at: