Designing Future Wheat Resources

Breeders Diversity Toolkit

  • The ‘Recommended List’ of DFW pre breeding lines.
  • Near Isogenic Lines under constant selection.
  • Best available molecular markers for target QTL.
  • The product delivered by DFW to commercial wheat breeding.
  • Led to the formation of a new post- the Derived Germplasm Specialist at the GRU

The aim of Designing Future Wheat (DFW) Programme is to deliver new useful genetic variation and knowledge to breeding. Pre-breeding germplasm is developed and handled in a collaboration between DFW WP3 and the Germplasm Resource Unit (GRU). The new pre-breeding germplasm will include near isogenic lines (NILs) from wheat diversity sources. NILs or equivalent material are assessed for the trait of interest within DFW.

Particularly promising germplasm will be multiplied and trialed in multi-site trials at commercial breeders and scientific institutions as an annually changing Breeders’ Diversity Toolkit (BTK) series. The DFW BTK series carries on from the WISP Breeders’ Diversity Toolkit (see below). It is one major result of DFW, taking output from WP1 and WP2 forward to pre-breeding. 

DFW Breeders’ Diversity Toolkit Selection Committee

Decisions on QTL or genomic regions and germplasm are made by the Breeders Toolkit Annual Selection Committee Meeting. It decides on the content of the DFW NIL back-crossing pipeline (NIL sets, putative candidates for a BTK) and on the final selection of the annual BTK lines.

The following representatives sit on the committee:

DFW_BTK_Selection_Committee_2021_02.2

QTL or genomic regions and germplasm can be nominated by any researcher within DFW to the chair of the committee by submitting a completed form on germplasm, trait of interest, markers etc. using the following form: BTK germplasm nomination form. The committee meets every year in February to decide on the larger back-crossing pipeline and in September to decide on the next BTK.

The BTK will be tested in multi-site trials at different breeding companies and institutions.

Summary of all accessions grown in BTK trials from 2017-2022

Download a spreadsheet with the accession names from all Breeders’ Toolkit trials up to 2022:

DFW Breeders’ Toolkit (BTK) 2021-2022

The accessions in the September 2021 selection are: a) introgressions of Synthetic Wheat into Robigus (WS); b) BC3 Near Isogenic Lines from mutant wheat into Paragon (WM); c) introgressions of Ae. Mutica (wild wheat relative) into Paragon (WR); e) Near Isogenic Lines from landrace cultivars in Paragon (WL). Not all lines are grown at all seven Breeders’ sites. Accessions and controls were planted in mostly three replicates in yield trials.

Type (Wheat)

Accession Name

Description

Recipent Cultivar

BTK Code

Breed. sites

WS SHW095-SEL 0454 donor NIAB SHW095 Robigus WBTK0049 7
WS SHW035-SEL 0302 donor NIAB SHW035 Robigus WBTK0050 7
WS SHW089-SEL 0385 donor NIAB SHW089 Robigus WBTK0051 7
WS SHW027-SEL 0279 donor NIAB SHW027 Robigus WBTK0052 7
WS SHW080-SEL 0435 donor NIAB SHW080 Robigus WBTK0053 7
WS SHW082-SEL 0338 donor NIAB SHW082 Robigus WBTK0054 7
WM GPC-B1_WT GW2-ABD_WT WT (BC3) Paragon WBTK0055 4
WM gpc-B1_Mut GW2-ABD_WT GPC-B1 funct Paragon WBTK0056 7
WM GPC-B1_WT gw2-ABD_Mut gw2-triple + GPC-B1 non-funct Paragon WBTK0057 4
WM gpc-B1_Mut gw2-ABD_Mut gw2-triple + GPC-B1 funct Paragon WBTK0058 7
WR NU DHF1-006 T.ae. x Ae. mutica BC DH Paragon WBTK0059 7
WR NU DHF1-011 T.ae. x Ae. mutica BC DH Paragon WBTK0060 4
WR NU DHF1-010 T.ae. x Ae. mutica BC DH Paragon WBTK0061 4
WR NU DHF1-008 T.ae. x Ae. mutica BC DH Paragon WBTK0062 4
WR NU DHF1-013 T.ae. x Ae. mutica BC DH Paragon WBTK0063 4
WL ParW292-54-8-2-39-Q5B-PH-W Wat292 QTL 5B-PH Paragon WBTK0064 4
WL ParW292-54-8-2-43-Q5B-PH-P Wat292 QTL 5B-PH Paragon WBTK0065 4
WL ParW475-31-4-6-3-Q5B-GW-W Wat475 QTL 5B-GW Paragon WBTK0066 4
WL ParW475-31-4-6-15-Q5B-GW-P Wat475 QTL 5B-GW Paragon WBTK0067 4
WL ParW387-15-13-2-6-Q4A-GW-W Wat387 QTL 4A-GW Paragon WBTK0068 4
WL ParW387-15-13-2-16-Q4A-GW-P Wat387 QTL 4A-GW Paragon WBTK0069 4
WL ParW496-41-12-4-14-Q2D-PH-W Wat496 QTL 2D-PH Paragon WBTK0070 4
WL ParW496-41-12-4-7-Q2D-PH-P Wat496 QTL 2D-PH Paragon WBTK0071 4
WL ParW141-4-5-1-15-Q6B-GY-W Wat141 QTL 6B-GY Paragon WBTK0072 4
WL ParW141-4-5-1-2-Q6B-GY-P Wat141 QTL 6B-GY Paragon WBTK0073 4
WL ParW199-6-9-6-21-Q6B-NDVI-W Wat199 QTL 6B-NDVI Paragon WBTK0074 4
WL ParW199-6-9-6-38-Q6B-NDVI-P Wat199 QTL 6B-NDVI Paragon WBTK0075 4
WL ParW032-78-7-14-10-Q4D-GY-W Wat032 QTL 4D-GY Paragon WBTK0076 4
WL ParW032-78-7-14-11-Q4D-GY-P Wat032 QTL 4D-GY Paragon WBTK0077 4
WL ParW468-34-12-7-19-Q6A-HI-W Wat468 QTL 6A-HI Paragon WBTK0078 1
WL ParW468-34-12-7-5-Q6A-HI-P Wat468 QTL 6A-HI Paragon WBTK0079 1
WL ParW199-22-5-4-10-Q2B-HI-W Wat199 QTL 2B-HI Paragon WBTK0080 1
WL ParW199-22-5-4-15-Q2B-HI-P Wat199 QTL 2B-HI Paragon WBTK0081 1
WL ParW034-13-2-7-17-Q2A-BM-W Wat034 QTL 2A-BM Paragon WBTK0082 1
WL ParW034-13-2-7-22-Q2A-BM-P Wat034 QTL 2A-BM Paragon WBTK0083 1
WL ParW398-88-13-5-9-Q6B-Hd-W Wat398 QTL 6B-Hd Paragon WBTK0084 1
WL ParW398-88-13-5-15-Q6B-Hd-P Wat398 QTL 6B-Hd Paragon WBTK0085 1
WL ParW209-42-4-2-16-Q6A-BM-W Wat209 QTL 6A-BM Paragon WBTK0086 1
WL ParW209-42-4-2-13-Q6A-BM-P Wat209 QTL 6A-BM Paragon WBTK0087 1
WL ParW292-91-1-3-18-Q3D-BM-W Wat292 QTL 3D-BM Paragon WBTK0088 1
WL ParW292-91-1-3-11-Q3D-BM-P Wat292 QTL 3D-BM Paragon WBTK0089 1
WL ParW246-83-4-5-7-Q1B-NDVI-W Wat246 QTL 1B-NDVI Paragon WBTK0090 1
WL ParW246-83-4-5-15-Q1B-NDVI-P Wat246 QTL 1B-NDVI Paragon WBTK0091 1
WL ParW199-59-6-8-12-Q7A-BM-W Wat199 QTL 7A-BM Paragon WBTK0092 1
WL ParW199-59-6-8-10-Q7A-BM-P Wat199 QTL 7A-BM Paragon WBTK0093 1
Control Paragon Spring Control WBTK-PAR1 7
Control Robigus Winter Control WBTK-ROB1 7
Control Santiago Winter Control WBTK-SAN1 7

DFW Breeders’ Toolkit (BTK) 2020-2021

Like last season, the accessions in the September 2020 selection: are a) introgressions of Durum, Emmer or Synthetic Wheat into Robigus (WS); b) one introgressions of Ae. Mutica (wild wheat relative) into Paragon (WR); c) Near Isogenic Lines from landrace cultivars in Paragon (WL); or e) Near Isogenic Lines from mutant wheat into Paragon (WM). Accessions and controls were planted in 3 replicates in yield trials.

Type (Wheat)

Accession Name

Description

Recipent Cultivar

GRU Code

WS

SHW-075Bc1 F6_SEL165

SHW-075Bc1 F6_SEL165

Robigus

WBTK0041

WS

SHW-008Bc1 F7_SEL63

SHW-008Bc1 F7_SEL63

Robigus

WBTK0040

WS

SHW-008Bc1 F7_SEL57

SHW-008Bc1 F7_SEL57

Robigus

WBTK0039

WS

SHW-008Bc1 F7_SEL50B

SHW-008Bc1 F7_SEL50B

Robigus

WBTK0038

WS

DFW SEL 0011B

DFW SEL 0011B

Robigus

WBTK0036

WS

DFW SEL 0015C

DFW SEL 0015C

Robigus

WBTK0036

WR

DHF1-196 T. aestivum x Ae. Mutica

Ae. mutica DH BC

Paragon

WBTK0046

WL

PW292-93-7-13-Q1B-MATU-W

Wat 1190292 QTL 1B-MAT

Paragon

WBTK0045

WL

PW292-93-7-16-Q1B-MATU-P

Wat 1190292 QTL 1B-MAT

Paragon

WBTK0044

WL

PW352-26-2-19-Q5D-MATU-P

Wat 1190352 QTL 5D-MAT

Paragon

WBTK0042

WL

PW352-26-2-2-Q5D-MATU-W

Wat 1190352 QTL 5D-MAT

Paragon

WBTK0043

WM

Paragon (BC4) (gw2-ABD_Mut)

TaGW2 triple knock-out

Paragon

WBTK0047

WM

Paragon (BC4) (GW2_ABD_WT)

TaGW2 wild-type

Paragon

WBTK0048

Control

Paragon

Spring Control

 

W10074

Control

Santiago

Winter Control, Yield Standard

 

W10338

Control

Robigus

Winter Control

 

W9999

BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU – BTK collection), Par=Paragon wild type, Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic.

DFW Breeders’ Toolkit (BTK) 2019-2020

The accessions in the September 2019 selections are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus; or introgressions of wild wheat relatives into Paragon; or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls were planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2019-20:

Type

(Wheat)

Accession Name

Description

of Introgression

Recipient

Cultivar

GRU

code

WL PW141-21-3-4-Q2A-GFP-W Wat 1190141 QTL 2A-GFP Paragon WBTK0020
WL PW292-25-6-3-Q2A-MATU-W Wat 1190292 QTL 2A-MATU Paragon WBTK0021
WL PW352-26-5-19-Q2A-DTMA-W Wat 1190352 QTL 2A-DTMA Paragon WBTK0022
WL PW468-84-4-2-Q5A-NDRE-W Wat 1190468 QTL 5A-NDRE Paragon WBTK0023
WS SHW008 SYN Sel 51 NIAB SHW-008 BC1F6 Robigus WBTK0024
WS SHW008 SYN Sel 61 NIAB SHW-008 BC1F6 Robigus WBTK0025
WS SHW090 SYN Sel 70 NIAB SHW-090 BC1F6 Robigus WBTK0026
WR D029 DHF1-29 Ae. mutica DH BC Paragon WBTK0027
WR D329 DHF1-329 Ae. mutica DH BC Paragon WBTK0028
WM gw2-A / Par (+/-) gw2-A Paragon WBTK0029
WM Par / POL (-/+) POL Paragon WBTK0030
WM gw2-A / POL (+/+) gw2-A and POL Paragon WBTK0031
Control Paragon Parent Spring Control   W10074
Control Robigus Parent Winter Control   W9999
Control Santiago Yield Standard Winter Control   W10338

Abbreviations:
BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU – BTK collection), gw2-A= grain weigth 2-A allele, Par=Paragon wild type, POL=T. polonicum P1 allele (grain length), Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic .

DFW Breeders’ Toolkit 2018-2019

The accessions in the current selection (September 2018) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2018 (GRU codes in brackets):

Accession Description GRU code
NIAB-TK-1746 Karim Durum Wheat introgression into Paragon WS0087
NIAB-TK-1549: Synthetic Hexaploid Wheat-18 introgression into Robigus WS0063
NIAB-TK-1604: Tios Emmer Landrace introgression into Robigus WS0076
PW34.12 Q1A-HT landrace allele from Watkins 1190034 WL0105
PW34.12 Q1A-HT comparative Paragon allele WL0104
PW468.84 Q5A GRYLD landrace allele from Watkins 1190468 WL0292
PW468.84 Q5A GRYLD comparative Paragon allele WL0291
Paragon, parental control
Robigus, parental control
Santiago, industry standard

 

DFW Breeders’ Diversity Toolkit 2017

The genotypes in the current selection (September 2017) are Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).

  • Information and statistics on the result of DFW BTK 2017 will be published here soon after harvest 2018.
  • Information and statistics on the result of WISP BTK 2016 and the new varieties in the DFW 2017 Toolkit: DFW_WP3_Breeders_Toolkit_2017.pdf

To obtain seed contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at simon.orford@jic.ac.uk

Normally, 500g of each line requested will be dispatched upon receipt of signed MTA. In the event of seed stock exhaustion, seed will be provided after regeneration at GRU.

Axiom Genotypes of NILs

Many of the NILs, candidates for the BTK, have been genotyped using the 35k bread wheat genotyping array. Genotypes can be inspected at: