Designing Future Wheat Resources

Breeders Diversity Toolkit

  • The ‘Recommended List’ of DFW pre breeding lines.
  • Near Isogenic Lines under constant selection.
  • Best available molecular markers for target QTL.
  • The product delivered by DFW to commercial wheat breeding.
  • Led to the formation of a new post- the Derived Germplasm Specialist at the GRU

The aim of Designing Future Wheat (DFW) Programme is to deliver new useful genetic variation and knowledge to breeding. Pre-breeding germplasm is developed and handled in a collaboration between DFW WP3 and the Germplasm Resource Unit (GRU). The new pre-breeding germplasm will include near isogenic lines (NILs) from wheat diversity sources. NILs or equivalent material are assessed for the trait of interest within DFW.

Particularly promising germplasm will be multiplied and trialed in multi-site trials at commercial breeders and scientific institutions as an annually changing Breeders’ Diversity Toolkit (BTK) series. The DFW BTK series carries on from the WISP Breeders’ Diversity Toolkit (see below). It is one major result of DFW, taking output from WP1 and WP2 forward to pre-breeding. 

DFW Breeders’ Diversity Toolkit Selection Committee

Decisions on QTL or genomic regions and germplasm are made by the Breeders Toolkit Annual Selection Committee Meeting. It decides on the content of the DFW NIL back-crossing pipeline (NIL sets, putative candidates for a BTK) and on the final selection of the annual BTK lines.

The following representatives sit on the committee:


QTL or genomic regions and germplasm can be nominated by any researcher within DFW to the chair of the committee by submitting a completed form on germplasm, trait of interest, markers etc. using the following form: BTK germplasm nomination form. The committee meets every year in February to decide on the larger back-crossing pipeline and in September to decide on the next BTK.

The BTK will be tested in multi-site trials at different breeding companies and institutions.

DFW Breeders’ Toolkit (BTK) 2019-2020

The accessions in the current selection (September 2019) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus; or introgressions of wild wheat relatives into Paragon; or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2019-20:



Accession Name


of Introgression





WL PW141-21-3-4-Q2A-GFP-W Wat 1190141 QTL 2A-GFP Paragon WBTK0020
WL PW292-25-6-3-Q2A-MATU-W Wat 1190292 QTL 2A-MATU Paragon WBTK0021
WL PW352-26-5-19-Q2A-DTMA-W Wat 1190352 QTL 2A-DTMA Paragon WBTK0022
WL PW468-84-4-2-Q5A-NDRE-W Wat 1190468 QTL 5A-NDRE Paragon WBTK0023
WS SHW008 SYN Sel 51 NIAB SHW-008 BC1F6 Robigus WBTK0024
WS SHW008 SYN Sel 61 NIAB SHW-008 BC1F6 Robigus WBTK0025
WS SHW090 SYN Sel 70 NIAB SHW-090 BC1F6 Robigus WBTK0026
WR D029 DHF1-29 Ae. mutica DH BC Paragon WBTK0027
WR D329 DHF1-329 Ae. mutica DH BC Paragon WBTK0028
WM gw2-A / Par (+/-) gw2-A Paragon WBTK0029
WM Par / POL (-/+) POL Paragon WBTK0030
WM gw2-A / POL (+/+) gw2-A and POL Paragon WBTK0031
Control Paragon Parent Spring Control   W10074
Control Robigus Parent Winter Control   W9999
Control Santiago Yield Standard Winter Control   W10338

BC=back cross, DH= doubled haploid, GRU= germplasm resource centre (GRU – BTK collection), gw2-A= grain weigth 2-A allele, Par=Paragon wild type, POL=T. polonicum P1 allele (grain length), Wat= Watkins landrace, WL= derived from wheat landrace, WM= derived from wheat mutant WR= derived from wheat wild relative, WS= derived from wheat synthetic .

DFW Breeders’ Toolkit 2018-2019

The accessions in the current selection (September 2018) are introgression of Durum, Emmer or Synthetic Wheat into Paragon or Robigus or Near Isogenic Lines from landrace cultivars in Paragon. Each accession and controls will be planted in 3 replicates in yield trials. The following accessions are in the DFW BTK 2018 (GRU codes in brackets):

Accession Description GRU code
NIAB-TK-1746 Karim Durum Wheat introgression into Paragon WS0087
NIAB-TK-1549: Synthetic Hexaploid Wheat-18 introgression into Robigus WS0063
NIAB-TK-1604: Tios Emmer Landrace introgression into Robigus WS0076
PW34.12 Q1A-HT landrace allele from Watkins 1190034 WL0105
PW34.12 Q1A-HT comparative Paragon allele WL0104
PW468.84 Q5A GRYLD landrace allele from Watkins 1190468 WL0292
PW468.84 Q5A GRYLD comparative Paragon allele WL0291
Paragon, parental control
Robigus, parental control
Santiago, industry standard


DFW Breeders’ Diversity Toolkit 2017

The genotypes in the current selection (September 2017) are Near Isogenic Lines either derived from landrace cultivars (two NIL streams from former WISP) or from an EMS mutant (one NIL stream from WP1).

  • Information and statistics on the result of DFW BTK 2017 will be published here soon after harvest 2018.
  • Information and statistics on the result of WISP BTK 2016 and the new varieties in the DFW 2017 Toolkit: DFW_WP3_Breeders_Toolkit_2017.pdf

To obtain seed contact Simon Orford, Germplasm Resources Unit, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH at

Normally, 500g of each line requested will be dispatched upon receipt of signed MTA. In the event of seed stock exhaustion, seed will be provided after regeneration at GRU.

Axiom Genotypes of NILs

Many of the NILs, candidates for the BTK, have been genotyped using the 35k bread wheat genotyping array. Genotypes can be inspected at: